Journal: Frontiers in Molecular Biosciences

Article Title: Identifying pyroptosis-related lncRNAs to predict prognosis and immune regulation in hepatocellular carcinoma

doi: 10.3389/fmolb.2026.1714966

Figure Lengend Snippet: Schematic showing the experimental layout of the study.

Article Snippet: The following antibodies were used in this stage: beta-tubulin (cat no. 10068-1-AP, 1:1,000, Proteintech, China); IL-18 (cat no. 10663-1-AP, 1:10,000); IL-1β (cat no. 16806-1-AP, 1:3,000); gasdermin D (GSDMD; cat no. 20770-1-AP, 1:2,000); caspase-1 (cat no. 22915-1-AP, 1:2,000/1:50, Proteintech, China); NLRP3 (cat no. 68102-1-Ig, 1:2,000/1:50, Proteintech, China); and CD3 (cat no. GB13440, 1:200, Servicebio, China).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Identifying pyroptosis-related lncRNAs to predict prognosis and immune regulation in hepatocellular carcinoma

doi: 10.3389/fmolb.2026.1714966

Figure Lengend Snippet: Prognostic value of the risk model based on the eight PRlncRNAs in the cancer genome atlas (TCGA) database. (A) Kaplan–Meier survival analysis of the high-risk and low-risk cohorts based on the risk model and median risk score. (B) Scatterplot based on the survival status of each sample, where the blue and orange dots represent survival and death, respectively. (C) Risk curve based on the risk score of each sample. (D) Forest plot for the univariate Cox regression analysis. (E) Forest plot for the multivariate Cox regression analysis. (F) Receiver operating characteristic (ROC) curves of the risk score and clinicopathological variables. (G) ROC curves and area under the curve (AUC) values at 1-, 3-, and 5-year survival for the predictive signature.

Article Snippet: The following antibodies were used in this stage: beta-tubulin (cat no. 10068-1-AP, 1:1,000, Proteintech, China); IL-18 (cat no. 10663-1-AP, 1:10,000); IL-1β (cat no. 16806-1-AP, 1:3,000); gasdermin D (GSDMD; cat no. 20770-1-AP, 1:2,000); caspase-1 (cat no. 22915-1-AP, 1:2,000/1:50, Proteintech, China); NLRP3 (cat no. 68102-1-Ig, 1:2,000/1:50, Proteintech, China); and CD3 (cat no. GB13440, 1:200, Servicebio, China).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Identifying pyroptosis-related lncRNAs to predict prognosis and immune regulation in hepatocellular carcinoma

doi: 10.3389/fmolb.2026.1714966

Figure Lengend Snippet: Verification of the prognostic model on the training and validation sets of TCGA database. (A) Kaplan–Meier survival analysis of the training set based on the high-risk and low-risk cohorts. (B) Kaplan–Meier survival analysis of the validation set based on the high-risk and low-risk cohorts. (C, D) ROC curves and AUCs at 1-, 3-, and 5-year survival for the training and validation sets. (E, F) Scatterplots based on the survival statuses of the samples from the training and validation sets, respectively. The blue and orange dots represent survival and death, respectively. (G, H) Risk curves based on the risk scores of the samples in the training and validation sets, respectively.

Article Snippet: The following antibodies were used in this stage: beta-tubulin (cat no. 10068-1-AP, 1:1,000, Proteintech, China); IL-18 (cat no. 10663-1-AP, 1:10,000); IL-1β (cat no. 16806-1-AP, 1:3,000); gasdermin D (GSDMD; cat no. 20770-1-AP, 1:2,000); caspase-1 (cat no. 22915-1-AP, 1:2,000/1:50, Proteintech, China); NLRP3 (cat no. 68102-1-Ig, 1:2,000/1:50, Proteintech, China); and CD3 (cat no. GB13440, 1:200, Servicebio, China).

Techniques: Biomarker Discovery

Journal: Frontiers in Molecular Biosciences

Article Title: Identifying pyroptosis-related lncRNAs to predict prognosis and immune regulation in hepatocellular carcinoma

doi: 10.3389/fmolb.2026.1714966

Figure Lengend Snippet: Effects of inhibiting the expression of LINC00942 on HCC cell proliferation progress. (A, B) Differential expressions of LINC00942 between cancer and non-carcinoma samples (n = 39). (C, D) Kaplan–Meier analysis of starBaseV3.0 ( http://starbase.sysu.edu.cn/ ) and clinical samples from Xijing Hospital. (E) Comparison of normal liver and HCC cells for verifying LINC00942 expressions. (F) LINC00942 is downregulated in MHCC-97H and Hep-3B cells using siRNAs. (G–I) Proliferation of MHCC-97H and Hep-3B cells transfected with siRNAs against LINC00942 measured using the CCK-8, EdU, and colony formation assays, respectively. (J, K) Transwell assays with and without the Matrigel matrix to assess the migration and invasion abilities of si-LINC00942. (L) RNA expression levels of NLRP3/caspase-1 after knockdown of LINC00942. (M) Changes in the proteins after knockdown of LINC00942 assessed by Western blotting. (N) Co-localization of CD3 and classical markers of pyroptosis based on multiplex immunohistochemical assay. The paraffin-embedded HCC samples were immunofluorescently stained for NLRP3 (red), caspase-1 (pink), and CD3 (green). “ns” indicates not significant, * p < 0.05, ** p < 0.01, and *** p < 0.005.

Article Snippet: The following antibodies were used in this stage: beta-tubulin (cat no. 10068-1-AP, 1:1,000, Proteintech, China); IL-18 (cat no. 10663-1-AP, 1:10,000); IL-1β (cat no. 16806-1-AP, 1:3,000); gasdermin D (GSDMD; cat no. 20770-1-AP, 1:2,000); caspase-1 (cat no. 22915-1-AP, 1:2,000/1:50, Proteintech, China); NLRP3 (cat no. 68102-1-Ig, 1:2,000/1:50, Proteintech, China); and CD3 (cat no. GB13440, 1:200, Servicebio, China).

Techniques: Expressing, Comparison, Transfection, CCK-8 Assay, Migration, RNA Expression, Knockdown, Western Blot, Multiplex Assay, Immunohistochemical staining, Staining

Journal: iScience

Article Title: MRPL37 promotes hepatocellular carcinoma progression through modulating mitochondrial energy metabolism

doi: 10.1016/j.isci.2025.114052

Figure Lengend Snippet: MRPL37 knockdown inhibits HCC cell proliferation, cell cycle, and induces apoptosis in vitro (A and B) qPCR and WB analysis showing the mRNA and protein levels of MRPL37 in JHH-7 and SNU-398 cells upon MRPL37 knockdown. (C) CCK-8 assay showing the effect of MRPL37 knockdown on cell proliferation in JHH-7 and SNU-398. (D and E) Colony formation assay showing the effect of MRPL37 knockdown on cell proliferation in JHH-7 and SNU-398. (F and G) Flow cytometry analysis of the cell cycle distribution in JHH-7 and SNU-398 cells after MRPL37 knockdown. (H and I) Flow cytometry analysis showing cell apoptosis in JHH-7 and SNU-398 cells after MRPL37 knockdown cells. (J) WB validates the restored MRPL37 expression upon MRPL37 knockdown in in JHH-7 and SNU-398 cells. (K) CCK-8 assay shows the effect of restored MRPL37 expression on cell proliferation upon MRPL37 knockdown in in JHH-7 and SNU-398 cells. Data in A, C, E, G, I, and K are presented as the mean ± SD ( n = 3, n represents number of biological replicates). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001, ns, not significant. Two-tailed Student’s t test, One-way ANOVA with Tukey’s test and two-way ANOVA with post-hoc tests.

Article Snippet: Annexin V-FITC/PI Cell Apoptosis Detection Kit , Servicebio , Cat# G1511.

Techniques: Knockdown, In Vitro, CCK-8 Assay, Colony Assay, Flow Cytometry, Expressing, Two Tailed Test